In the future, I wish to pursue a Doctor of Philosophy (PhD) in Immunology; and this project relates to my overall goal because the research methods overlap with what a researcher will be performing in the field of immunology such as polymerase chain reactions for DNA synthesis and growing eukaryotic cell lines. The difference is that this project observes the effect of treatment on the bacterium while immunology focuses on the effect of the treatment on host cells.
On a weekly basis, I perform many different types of experiments based on what is needed for the paper that I am writing for publication. The process usually includes researching how to perform certain experiments by looking through publications and creating a mode of action to create the most successful outcome for the experiment. After creating a method, I gather all the materials and disinfect everything to ensure a sterile environment without contamination from outside bacteria. After the experiment has been performed, the data is interpreted through programs such as GraphPad and Excel. This week, through experimentation, I found that the cranberry proanthocyanidins have the ability to block motility of P. aeruginosa and this is a possible mechanism as to why the bacteria cannot communicate with each other and form a biofilm.