In brief, my project consists of 4 main experiments leading to the Nitric Oxide Assay that will evaluate the levels of toxicity. First, I have to grow cells in an incubator. After at least 3-4 days, I will have enough alveoli macrophages to plate them in a 96-well plate. Twenty four hours later, I have to synthesize 7 ligands that I will use as conditions to assess the levels of toxicity associated with Surfactant Protein A and Carbon Nanotubes on the cells. Consequently, I have to add the ligands to the cells in the plate. The next day, the cells are ready to be tested in a Nitric Oxide Assay that measure the light absorbance to analyze the toxicity associated with each condition. the The entire experiment takes about a week and a half to complete. In order to affirm the results and to confirm reproducibility, I have conducted the experiment 3 times already and gotten very similar results.
Moreover, this project is preparing for medical school by becoming expose to laboratory research and being able to handle school work and a job with great results on both sides. My dream is to become a surgeon and doing the cell culture experiment, the ligand addition, and the nitric oxide assays are helping becoming very skillful with my hands. Pipetting is like an art and everyday I am becoming more competent. As a doctor I have to be as meticulous and responsible as I am with this experiment because the stakes are higher and human lives are on the line. Furthermore, the next phase of the project is to publish the results. I have started doing the a review of recent works related to my project. Getting exposed to other scientific works and helping write this paper will provide me the opportunity of being a published author and also be able to present my research my many conferences nationally and internationally.