On a weekly basis, my colleague and I perform
the procedures outlined in our proposals. At the beginning of the semester, we
began ordering supplies and materials needed as well as conducting workbench
organization. Primers were ordered in preparation for performing PCR after
extracting the DNA from our mycobacteriophage. We were able to obtain control
mycobacteriophages from a JMU collaborator a week ago, in order to test our
ordered primers. However this week, my colleague and I made the mistake of
placing our control mycobacteriophages in the deep freezer. This can destroy
the mycobacteriophage organisms in the samples. We have taken them out and are
now conducting the standard amplification procedures to help us identify if our
mycobacteriophage are still viable or not. If mycobacteriophages are found, with
the infection of mycobacterium smegmatis, we can continue performing PCR and
testing the validity of our primers. If not, then we will have to wait another
week for another order of control phages.