This semester I
conducted research on the effect of lead on metabotropic glutamatergic
receptors in the brain. It is widely known that chronic exposure to lead can
cause serious neurological damage, potentially causing deficits in cognitive
development. The goal of my research was to detail the specific mechanism in
which lead affects our brains. Lead targets specific receptors in our brains
that control the transmission of glutamate, a neurotransmitter that is vital in
cognitive development. The specific receptor my project focuses on is the
mGluR7a metabotropic glutamatergic receptor. To test the function of this
receptor, Xenopus Laevis oocytes were used as a model system.
The first objective of
my project was to build a fully functioning electrophysiology lab where I could
conduct the experiment. This involved purchasing and setting up microscopes,
displays, a perfusion-vacuum system, and a two-electrode voltage clamp system.
A variety of electrophysiology solutions were also made to be used later on.
Prior to actual experimentation, I practiced techniques such as injecting the
oocytes to help me during the actual experiment. These skills and materials
were to be used to begin recording data and measuring the response of these
oocytes in the presence of lead.
However, before any
conclusive data was obtained, COVID-19 forced us to stop our work in the lab.
Eager to continue my project, I worked with my mentor Dr. Greta Ann Herin, to
find a way to continue researching my topic. Dr. Herin provided me with
existing data that gave me a look at how lead impairs the function of these
receptors in oocytes. This allowed me to reach a conclusion that lead does
indeed impair these glutamatergic receptors in a concentration-dependent
manner.
Overall, my URSP
experience allowed me to build on a critical skill in the field of research,
which is adaptability. Despite the COVID-19 crisis, I was still able to
continue my project and present my research. Moving forward I hope to soon be
able to get back into the lab and continue the research process.
